Prince Harry has been accused of "harassment and bullying at scale" by the chair of Sentebale, the charity he co-founded.
Dr Sophie Chandauka made the allegations following the Duke of Sussex's departure from the organisation earlier this week.
Dr Chandauka claimed Prince Harry "authorised the release of a damaging piece of news" without informing her or other directors.
"That is an example of harassment and bullying at scale," she told Sky News and accused the Duke of "unleashing the Sussex PR machine" against her.
Prince Harry and Prince Seeiso of Lesotho released a joint statement on Tuesday saying they had stepped down "in support of and solidarity with" the board of trustees.
They stated their relationship with the chairwoman had "broken down beyond repair, creating an untenable situation."
The problems reportedly stem from a decision to focus fundraising in Africa.
A source close to the former trustees has described Dr Chandauka's allegations as "completely baseless".
They denied that she was bullied or harassed, or that the "Sussex machine" was "unleashed" on her.
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In an exclusive poll for GB News membership readers, a more than two-thirds majority (68 per cent) of the 494 voters thought negative press around Prince Harry does not damage the Royal Family, while 32 per cent thought it still does.
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